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  4. Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

  • 中文名稱：
    小鼠抗雙鏈DNA抗體/天然DNA抗體(IgM)酶聯免疫試劑盒
  • 貨號：
    CSB-E12911m
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠抗雙鏈DNA抗體/天然DNA抗體(IgM)酶聯免疫試劑盒（CSB-E12911m）為捕獲法ELISA試劑盒，定量檢測血清、血漿樣本中的dsDNA Ab (IgM)含量。dsDNA Ab (IgM) 即抗雙鏈DNA抗體（IgM類）。在系統性紅斑狼瘡等自身免疫病中，機體免疫系統失調產生該抗體，它可與體內雙鏈DNA結合形成免疫復合物，沉積在組織器官引發炎癥損傷。深入研究其作用機制，對疾病診斷和治療有重要意義。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產品可廣泛應用于自身免疫疾病機制研究、藥物開發中抗體水平動態監測、免疫調控實驗評估等科研場景，為動物模型中的免疫應答分析及病理機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    dsDNA Ab (IgM)
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Capture ELISA
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %85-105
    1:2Average %89
    Range %85-98
    1:4Average %93
    Range %85-97
    1:8Average %95
    Range %89-110
  • 回收率：
    The recovery of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9483-101
    EDTA plasma (n=4)9787-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.501 2.531 2.516 2.377
    502.178 2.203 2.191 2.052
    251.803 1.822 1.813 1.674
    12.51.254 1.268 1.261 1.122
    6.250.731 0.745 0.738 0.599
    3.120.405 0.397 0.401 0.262
    1.560.255 0.249 0.252 0.113
    00.138 0.140 0.139
  • 數據處理：
  • 貨期：
    3-5 working days

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