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  4. Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名稱：
    小鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯免疫試劑盒
  • 貨號：
    CSB-E10787m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯免疫試劑盒（CSB-E10787m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Aβ1-42含量。Aβ1-42是重要的阿爾茨海默病相關靶點。在患者腦內該蛋白異常聚集形成淀粉樣斑塊，破壞神經細胞功能與結構。研究聚焦其產生、聚集過程及與神經毒性的關聯，旨在通過抑制生成、促進清除等機制來改善病情、研發治療藥物。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于阿爾茨海默病小鼠模型構建、Aβ病理機制研究、藥物干預效果評估等實驗場景，為神經科學領域提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 縮寫：
    Aβ1-42
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-99  
    1:2 Average % 95  
    Range % 85-105  
    1:4 Average % 96  
    Range % 90-105  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 93 85-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.490 2.451 2.471 2.337  
    500 2.049 1.985 2.017 1.883  
    250 1.357 1.385 1.371 1.237  
    125 0.866 0.837 0.852 0.718  
    62.5 0.517 0.524 0.521 0.387  
    31.2 0.347 0.355 0.351 0.217  
    15.6 0.244 0.228 0.236 0.102  
    0 0.135 0.132 0.134 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

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相關問答

■ 常見問題解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a 96T sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.

靶點詳情

  • 最新研究進展：
    Aβ1-42是β淀粉樣蛋白（Aβ）家族的一員，它是阿爾茨海默病（AD）病理學的主要成分。最近的研究表明，Aβ1-42的積聚是導致AD的主要因素之一。已經發現許多抗Aβ1-42藥物，如免疫療法和β-淀粉樣蛋白藥物，這些藥物能夠清除Aβ1-42，并恢復AD患者的認知能力。此外，一些研究還發現，Aβ1-42可能是癲癇的重要病理因素，這為癲癇治療提供了新的思路。


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