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  4. Mouse Semaphorin-3F(SEMA3F) ELISA kit

Mouse Semaphorin-3F(SEMA3F) ELISA kit

  • 中文名稱：
    小鼠臂板蛋白-3F(SEMA3F)酶聯免疫試劑盒
  • 貨號：
    CSB-EL020985MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠臂板蛋白-3F(SEMA3F)酶聯免疫試劑盒（CSB-EL020985MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SEMA3F含量。SEMA3F是一種在細胞運動和粘附中發揮作用的蛋白，參與細胞動力學和神經系統發育。其研究背景涉及其在細胞運動和粘附中的調節機制，以及作為候選抑癌基因在腫瘤中的作用。研究機制主要圍繞其與受體結合，調節瘤細胞和內皮細胞功能，影響腫瘤生長和脈管生成。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，支持跨物種交叉反應驗證，適用于探索SEMA3F在神經系統疾病模型、腫瘤侵襲性研究或炎癥相關實驗中的動態表達特征本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Sema3f ELISA Kit; Semaphorin-3F ELISA Kit; Semaphorin IV ELISA Kit; Sema IV ELISA Kit
  • 縮寫：
    SEMA3F
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 87-97
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 101
    Range % 96-106
  • 回收率：
    The recovery of mouse SEMA3F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 88-94
    EDTA plasma (n=4) 107 103-111
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.567 2.699 2.633 2.529
    10 2.144 2.244 2.194 2.090
    5 1.569 1.625 1.597 1.493
    2.5 1.025 0.989 1.007 0.903
    1.25 0.748 0.746 0.747 0.643
    0.625 0.462 0.454 0.458 0.354
    0.312 0.210 0.223 0.217 0.113
    0 0.103 0.105 0.104 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 基因功能參考文獻：
    1. data suggest that Sema3f and Nrp2 are dispensable for the exclusion of vessels from the cap mesenchyme during kidney development. PMID: 28929539
    2. Sema3f is suppressed in outer nuclear layer in very low-density lipid-receptor deficient retina. PMID: 28373097
    3. Sema3F functions as an unconventional Sema3 that promotes extraembryonic angiogenesis by inhibiting the Myc-regulated synthesis of Thbs1 in visceral yolk sac epithelial cells. PMID: 28729362
    4. Nrp2 deficiency increased blood vessel permeability and decreased lymphatic vessel drainage during inflammation, highlighting the importance of the NRP2/SEMA3F pathway in the modulation of tissue swelling and resolution of postinflammatory edema. PMID: 27751443
    5. Sema3F plays important roles in the development of neuronal circuitry underlying the regulation of some aspects of anxiety and fear responses. PMID: 26856818
    6. The authors demonstrate that expression of Semaphorin-3F in the outer hair cell region inhibits type I spiral ganglion neuron process extension by activating Neuropilin-2 receptors expressed on spiral ganglion neurons. PMID: 26302206
    7. the embryonically developed wiring deficits in Sema3F mutants persist until adulthood PMID: 25874621
    8. Sema4A promotes Th1 and Th17 differentiation and increases adhesive activation of T cells to endothelial cells, even in the presence of IFN-beta. PMID: 24439904
    9. Sema3F is secreted by early-arriving axons of olfactory sensory neurons and is deposited at the anterodorsal olfactory bulb to repel Nrp2-positive axons that arrive later. PMID: 20550939
    10. These data suggest that Sema3F plays a relatively restricted, if any, role in its receptor expression and postnatal development of the somatosensory cortex, the cerebellum, and the main olfactory system. PMID: 20006658
    11. Sema3F is the principal ligand for Npn-2-mediated axon guidance events in vivo and is a critical determinant of limbic and peripheral nervous system circuitry PMID: 12890759
    12. Sema3F alternatively spliced isoforms share common axonal chemorepulsive properties and binding to neuropilin receptors, and are found to regulate endothelial cell morphology by remodeling lamellipodial protrusions. PMID: 14572462
    13. We describe a related signaling pathway involving secreted class 3 semaphorins, neuropilins, and a plexin receptor, PlexinD1, expressed by endothelial cells PMID: 15239958
    14. Using sema3F-/- mice, we show that Sema3F is critical for vomeronasal sensory neuron axonal fasciculation and for segregation of these sensory afferents from the main olfactory system and lamina-specific targeting of olfactory sensory axons PMID: 15483127
    15. Npn2/Sema3f signaling guides neural crest migration through the somite. PMID: 16319111
    16. Genetic evidence for a Nrp2-Sema3f interaction as a determinant of the wiring of axons of gucy2d+ neurons into the unusual configuration of necklace glomeruli. PMID: 17942483
    17. An important regulator of the ventral tangential cell migration stream, confining the migrating neurons on the telencephalon surface by repelling from the deeper domain. PMID: 18434520
    18. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001
    19. Semaphorin3F induces significant growth cone collapse of temporal, but not nasal, RGCs expressing high levels of Neuropilin-2. PMID: 18942144
    20. Data demonstrate that methyl-CpG binding protein 2 deficiency disrupts the establishment of neural connections before synaptogenesis by altering semaphorin 3F function. PMID: 19628041
    21. Soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. PMID: 19790074

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 組織特異性：
    Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.
  • 數據庫鏈接：