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  4. Mouse Lymphotoxin-beta(LTB) ELISA kit

Mouse Lymphotoxin-beta(LTB) ELISA kit

  • 中文名稱：
    小鼠淋巴毒素β(LTB)酶聯免疫試劑盒
  • 貨號：
    CSB-EL013220MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠淋巴毒素β(LTB)酶聯免疫試劑盒（CSB-EL013220MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LTB含量。靶點研究是藥物研發的關鍵，旨在發現疾病過程中的特定分子或細胞結構，通過藥物相互作用產生治療效果。研究機制涉及基因組學、蛋白質組學、生物信息學和高通量篩選，旨在精準識別疾病靶點，開發高效藥物。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，可滿足科研場景中對LTB的定量分析需求，如免疫相關疾病模型研究、炎癥信號通路探索或藥物干預實驗中生物標志物動態監測，為探究LTB在免疫調控中的功能機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Ltb; Tnfc; Tnfsf3; Lymphotoxin-beta; LT-beta; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LTB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-94
    1:2Average %84
    Range %80-88
    1:4Average %89
    Range %84-93
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of mouse LTB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-99
    EDTA plasma (n=4)104100-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.704 2.527 2.616 2.466
    4002.047 2.206 2.127 1.977
    2001.415 1.504 1.460 1.310
    1000.704 0.735 0.720 0.570
    500.394 0.419 0.407 0.257
    250.291 0.279 0.285 0.135
    12.50.213 0.205 0.209 0.059
    00.154 0.145 0.150
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface.
  • 基因功能參考文獻：
    1. The expression of lymphotoxin (LT)-B was significantly downregulated in the absence of T cells from nude mice and was recovered after the transfusion of T cells. PMID: 25266629
    2. TNF is able to upregulate LT-beta expression in hepatic cells at the transcriptional level by the binding of NF-kappaB p50/p65 heterodimers and Ets1 to their respective sites in the LT-beta promoter. PMID: 22742857
    3. Lymphotoxin-beta-receptor (LTbetaR) signaling, a pathway essential for lymphoid organogenesis, abrogates tertiary lymphoid organ development in heart transplantation. PMID: 21926237
    4. LTbeta RNA is detectable in embryos ranging from 5.5 to 18.5 days of development, e.g., in peripheral lymph nodes, Peyer's patches, thymus and skin of the E18.5 embryo and fetal liver of E12.5. PMID: 11994460
    5. membrane lymphotoxin beta contributes to anti-leishmanial immunity by controlling structural integrity of lymphoid organs PMID: 12115620
    6. The organogenic function of B-LTbeta is almost entirely restricted to spleen, where it supports the correct lymphoid architecture that is critical for an effective humoral immune response. PMID: 12354378
    7. microenvironment in peripheral lymphoid organs associated with lymphotoxin alpha/lymphotoxin beta-lymphotoxin beta receptor signaling and chemokine production is critical for recruitment efficiency of dendritic cel PMID: 12560241
    8. membrane LT-beta is important in resistance to Theiler's virus infection PMID: 12882833
    9. Lymphotoxin-mediated adhesion molecule expression may be important in the development of graft-versus-host skin disease. PMID: 14734744
    10. LTbeta, LTbeta receptor, and IFNgamma are involved in oval cell-mediated, but not hepatocyte-mediated, liver regeneration, and the absence of these pathways impairs the oval cell-dependent regenerative response. PMID: 15660390
    11. The lymphotoxin beta signaling pathway is an essential effector pathway for host defense against the beta-herpesvirus muromegalovirus (MCMV). PMID: 15905567
    12. Data show that, as an ectodysplasin target, lymphotoxin-beta regulates hair phenotype in developing hair follicles. PMID: 16738056
    13. Expression of LTbeta on lymphocytes enhances the induction of immune responses against limiting amounts of antigen PMID: 16841297
    14. Expression of lymphotoxin-alphabeta on antigen-specific T cells is required for DC function. PMID: 17452522
    15. Activation of LTbetaR by LTalphabeta mainly expressed on T lymphocytes is crucial for the down regulation of the inflammatory response in this experimental model. PMID: 17590442
    16. LTbeta shows a primary early function in periderm differentiation, with later transient effects on epidermal and hair follicle differentiation. PMID: 17673451
    17. signal was essential for primary B cell cluster formation with initial differentiation of follicular dendritic cells (FDCs) in neonatal mice. PMID: 17982070
    18. LTbetaR on hepatic stellate cells may be involved in signaling with LTbeta-expressing liver progenitor cells mediating recruitment of progenitor cells, hepatic stellate cells, & leukocytes for wound healing & regeneration during chronic liver injury. PMID: 19111021

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  • 亞細胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數據庫鏈接：

    KEGG: mmu:16994

    STRING: 10090.ENSMUSP00000025262

    UniGene: Mm.1715