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  4. Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

  • 中文名稱：
    小鼠組蛋白去乙酰化酶復(fù)合物亞基SAP130(SAP130)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020694MO
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    46.88 pg/mL-3000 pg/mL
  • 靈敏度：
    11.72 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠組蛋白去乙酰化酶復(fù)合物亞基SAP130(SAP130)酶聯(lián)免疫試劑盒（CSB-EL020694MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SAP130含量。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL，本產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如探究表觀遺傳調(diào)控機(jī)制、建立炎癥相關(guān)疾病動物模型、評估藥物干預(yù)對SAP130表達(dá)的影響等研究場景，為探索SAP130在免疫調(diào)控和疾病發(fā)生中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sap130 ELISA Kit; Histone deacetylase complex subunit SAP130 ELISA Kit; 130 kDa Sin3-associated polypeptide ELISA Kit; Sin3-associated polypeptide p130 ELISA Kit
  • 縮寫：
    SAP130
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAP130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %85-99
    1:2Average %98
    Range %93-102
    1:4Average %93
    Range %89-98
    1:8Average %91
    Range %87-97
  • 回收率：
    The recovery of mouse SAP130 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9493-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.622 2.535 2.579 2.430
    15002.117 2.154 2.136 1.987
    7501.402 1.520 1.461 1.312
    3750.736 0.780 0.758 0.609
    187.50.478 0.495 0.487 0.338
    93.750.311 0.316 0.314 0.165
    46.880.245 0.240 0.243 0.094
    00.153 0.144 0.149
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
4.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 我使用CSB-EL020694MO檢測sham組和mcao組小鼠外周血清樣本，樣本并非現(xiàn)用現(xiàn)提，這可能導(dǎo)致sham組出線負(fù)數(shù)值，也有可能是試劑盒靈敏度不太夠，實(shí)驗(yàn)組數(shù)值趨勢和預(yù)計(jì)指標(biāo)相似。簡直說明書添加結(jié)果計(jì)算示例，否則第一次做elisa的同學(xué)可能不會計(jì)算

By 付老師

靶點(diǎn)詳情

  • 功能：
    Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    SAP130 family
  • 數(shù)據(jù)庫鏈接：