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  4. Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

  • 中文名稱：
    小鼠GDNF家族α樣受體(GFRAL)酶聯免疫試劑盒
  • 貨號：
    CSB-EL009383MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠GDNF家族α樣受體(GFRAL)酶聯免疫試劑盒（CSB-EL009383MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的GFRAL含量。GFRAL是一個重要靶點。它與機體能量代謝等生理過程密切相關。研究機制上，其能參與特定信號通路，可能成為調節能量平衡的關鍵環節。目前圍繞它正開展眾多科研工作，有望為肥胖癥等代謝疾病治療帶來新策略。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，本試劑盒通過高特異性配對抗體實現目標蛋白的精準捕獲與信號放大，適用于基礎科研領域探索GFRAL在代謝性疾病動物模型中的動態變化、評估特定組織或細胞中受體表達差異，以及篩選調控GFRAL通路的藥物或生物活性分子。產品嚴格驗證了線性范圍與靈敏度指標，可滿足體外實驗中低豐度蛋白的穩定檢測需求，為代謝機制研究、神經內分泌調控及疾病模型構建提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-98
    1:2 Average % 101
    Range % 97-107
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 88 84-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.859 1.952 1.906 1.766
    800 1.258 1.260 1.259 1.119
    400 0.720 0.734 0.727 0.587
    200 0.466 0.489 0.478 0.338
    100 0.315 0.328 0.322 0.182
    50 0.229 0.235 0.232 0.092
    25 0.172 0.176 0.174 0.034
    0 0.138 0.141 0.140 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
  • 基因功能參考文獻：
    1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein; Extracellular side.
  • 蛋白家族：
    GDNFR family
  • 組織特異性：
    Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
  • 數據庫鏈接：

    KEGG: mmu:404194

    UniGene: Mm.213862



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