Mouse Eotaxin 3 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠嗜酸粒細(xì)胞趨化蛋白Eotaxin 3(Eotaxin 3)酶聯(lián)免疫試劑盒（CSB-E15062m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Eotaxin 3含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于研究炎癥性疾病模型中嗜酸性粒細(xì)胞募集機(jī)制、評(píng)估實(shí)驗(yàn)性抗過敏藥物對(duì)趨化因子表達(dá)的調(diào)控作用，或解析免疫細(xì)胞與微環(huán)境相互作用的分子基礎(chǔ)，為呼吸道疾病、皮膚過敏等病理機(jī)制研究提供可靠工具。試劑盒操作流程標(biāo)準(zhǔn)化，適用于體外實(shí)驗(yàn)環(huán)境下多種生物樣本的平行檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Eotaxin 3
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Eotaxin 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-93
1:2	Average %	96
	Range %	92-100
1:4	Average %	103
	Range %	101-105
1:8	Average %	89
	Range %	84-94

回收率：

The recovery of mouse Eotaxin 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-94
EDTA plasma (n=4)	89	85-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.