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  4. Mouse Complement fragment 4a,C4a ELISA Kit

Mouse Complement fragment 4a,C4a ELISA Kit

  • 中文名稱：
    小鼠過(guò)敏毒素/補(bǔ)體片斷4a(C4a)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08517m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠過(guò)敏毒素/補(bǔ)體片斷4a(C4a)酶聯(lián)免疫試劑盒（CSB-E08517m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C4a含量。試劑盒檢測(cè)范圍為39 pg/mL-2500 pg/mL，適用于基礎(chǔ)免疫學(xué)研究，如炎癥性疾病動(dòng)物模型中補(bǔ)體激活程度的動(dòng)態(tài)監(jiān)測(cè)、藥物干預(yù)對(duì)補(bǔ)體系統(tǒng)影響的評(píng)估，或免疫細(xì)胞與補(bǔ)體互作機(jī)制等實(shí)驗(yàn)場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    C4a
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 96  
    Range % 87-105  
    1:2000 Average % 90  
    Range % 84-100  
    1:4000 Average % 95  
    Range % 82-103  
    1:8000 Average % 96  
    Range % 84-103  
  • 回收率：
    The recovery of mouse C4a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 87-105  
    EDTA plasma (n=4) 96 82-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2500 2.786 2.687 2.737 2.599  
    1250 2.136 2.042 2.089 1.951  
    625 1.253 1.265 1.259 1.121  
    312 0.784 0.752 0.768 0.630  
    156 0.543 0.521 0.532 0.394  
    78 0.364 0.342 0.353 0.215  
    39 0.242 0.239 0.241 0.103  
    0 0.136 0.140 0.138    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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