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  4. Mouse Ceruloplasmin,CP/CER ELISA Kit

Mouse Ceruloplasmin,CP/CER ELISA Kit

  • 中文名稱：
    小鼠銅藍蛋白(CP/CER)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07023m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠銅藍蛋白(CP/CER)酶聯(lián)免疫試劑盒（CSB-E07023m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CP含量。小鼠銅藍蛋白（CP/CER）是一種多銅氧化酶和抗氧化劑，主要在肝臟中合成，參與銅和鐵的代謝平衡，具有抗氧化活性。研究發(fā)現(xiàn)，銅藍蛋白在阿爾茨海默癥、帕金森病等神經(jīng)退行性疾病及糖尿病、肥胖等代謝性疾病中發(fā)揮重要作用，其功能可能與促進神經(jīng)細(xì)胞生長、改善學(xué)習(xí)記憶能力、調(diào)節(jié)脂質(zhì)代謝和抗氧化應(yīng)激有關(guān)。試劑盒檢測范圍為31.2 ng/mL-2000 ng/mL，適用于體外研究，可用于銅代謝機制探索、肝臟功能評估、炎癥性疾病模型建立等科研領(lǐng)域，支持小鼠血清（1:10稀釋）和細(xì)胞上清（直接檢測）等多種樣本類型。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cp ELISA Kit; Ceruloplasmin ELISA Kit; EC 1.16.3.1 ELISA Kit; Ferroxidase ELISA Kit
  • 縮寫：
    CP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    31.2 ng/mL-2000 ng/mL
  • 靈敏度：
    7.8 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 95  
    Range % 83-106  
    1:2000 Average % 92  
    Range % 83-104  
    1:4000 Average % 95  
    Range % 91-102  
    1:8000 Average % 93  
    Range % 87-99  
  • 回收率：
    The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 92 85-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.103 0.105 0.104    
    1000 0.169 0.171 0.170    
    500 0.301 0.309 0.305    
    250 0.575 0.582 0.579    
    125 1.294 1.306 1.300    
    62.5 1.855 1.864 1.860    
    31.2 2.536 2.541 2.539    
    0 2.879 2.887 2.883    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense.
  • 基因功能參考文獻：
    1. We thus used hephaestin (Heph) and ceruloplasmin (Cp) single-knockout mice and Heph/Cp double-knockout mice to investigate the roles of multicopper ferroxidases(MCF) in pancreatic iron homeostasis. We found that both HEPH and CP were expressed in the mouse pancreas, and that ablation of either MCF had limited effect on the pancreatic iron levels. PMID: 29883959
    2. Hephaestin and ceruloplasmin have roles in facilitating iron metabolism in the mouse kidney PMID: 27991585
    3. In D-galactosamine-sensitized mice CP+Cu(II) increased the LPS-induced lethality from 54 to 100%, while administration of antibodies against MIF prevented the lethal effect. The enhancement by CP+Cu(II) of the pro-inflammatory signal of MIF is discussed PMID: 26091949
    4. mice with mutation of Cp and Heph, iron accumulates in glia, while neurons have low iron levels. Both neurons and glia degenerate and mice become ataxic unless given an iron chelator. PMID: 26303407
    5. Data (including data from studies in knockout mice) suggest that ceruloplasmin and hephaestin play distinct roles in regulation of gene expression in various regions of the brain and are involved in iron homeostasis. PMID: 25788583
    6. an increase in Cp expression could contribute to tissue damage in early experimental autoimmune encephalomyelitis PMID: 24615902
    7. Evidence supports a regulatory role of both proteins (Ceruloplasmin (CP) and beta-amyloid protein precursor (APP)) in defence against iron-induced oxidative damage after TBI, which presents as a tractable therapeutic target. PMID: 24509156
    8. Genetic interactions between Cp, Mon1a, and the Slc40a1 locus are involved in iron metabolism. PMID: 24121729
    9. ceruloplasmin should provide a protective shield against inadvertent oxidant production by myeloperoxidase during inflammation PMID: 23306200
    10. The mouse ceruloplasmin gene has been mapped to chromosome 3. PMID: 178338
    11. Data found an increase in ceruloplasmin levels in the plasma of Npc1 -/- mice compared to Npc1 +/+ mice, and this increase was statistically significant (*p < 0.05). PMID: 22526561
    12. Cp and Heph are necessary for iron export from the retina but are not essential for iron import into the retina. PMID: 22342521
    13. levels of 5HT and norepinephrine and the expression of BDNF and its receptor trkB, are significantly reduced in the hippocampus of ceruloplasmin knockout mice PMID: 22035068
    14. Ceruloplasmin deficiency reduces levels of iron and BDNF in the cortex and striatum of young mice and increases their vulnerability to stroke. PMID: 21949858
    15. Ceruloplasmin stimulation of reelin cleavage is in line with a possible role of ceruloplasmin in nervous system development. PMID: 20188154
    16. Reduction in Cp is a sensitive biomarker for copper deficiency. PMID: 20170749
    17. mainly acts to release iron from cells in the liver PMID: 12393173
    18. Glycosylphosphatidylinositol-anchored ceruloplasmin is required for iron efflux from cells in the central nervous system. PMID: 12743117
    19. Cp has a pro-aggregative action on newly differentiated neurons in vitro specific for Cp in a concentration-dependent and saturable fashion. PMID: 12946701
    20. The copper-containing yeast protein Fet3p can restore iron homeostasis in phlebotomized mice with a deletion of the ceruloplasmin gene. PMID: 14739215
    21. Ceruloplasmin ane hephaestin are critical for CNS iron homeostasis and that loss of Cp and Heph in the mouse leads to age-dependent retinal neurodegeneration. PMID: 15365174
    22. Iron accumulation is reflected in increased ferritin expression in ceruloplasmin deficient mice and is accompanied by cell loss. PMID: 16988052
    23. the concentrations of ceruloplasmin and lactoferrin in milk were increased fivefold and more than 38-fold, respectively, within 48 h after weaning PMID: 16989572
    24. Transcripts of ceruloplasmin, involved in iron efflux, were overexpressed in periportal areas and the result was confirmed by in situ hybridization study. PMID: 18222182
    25. ROS affects RNA-protein complex formation to promote Cp mRNA decay. PMID: 19019832
    26. Datan confirmed that mouse ceruloplasmin had ferroxidase activity but revealed an additional ferroxidase in ceruloplasmin knockout mouse plasma. PMID: 19076073
    27. Microglial and endothelial induction of Cp is a neuroprotective mechanism during inflammation because Cp-deficient mice exhibited increased iron accumulation and demyelination when exposed to LPS and neurovascular reactivity to pneumococcal meningitis PMID: 17375196
    28. To elucidate the role of ceruloplasmin in copper metabolism, the kinetics of copper absorption, transport, distribution, and excretion were examined utilizing (64)Cu in wild-type and aceruloplasminemic mice PMID: 11461924

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Multicopper oxidase family
  • 組織特異性：
    Expressed in many tissues, including liver, eye and brain.
  • 數(shù)據(jù)庫鏈接：


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