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Mouse C-telopeptide of type Ⅱ collagen(CTX-Ⅱ) ELISA Kit

  • 中文名稱:
    小鼠Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯免疫試劑盒
  • 貨號:
    CSB-E16211m
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯免疫試劑盒(CSB-E16211m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL,在骨關節炎模型研究、軟骨退行性病變機制探索及藥物干預效果評估中具有重要應用價值;支持科研人員高效完成軟骨代謝相關疾病的動物模型構建、病理機制分析以及潛在治療靶點的驗證工作;為關節炎、骨質疏松等骨骼系統疾病的分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    C-telopeptide of type 2 collagen(CTX-2),_x000D_ C-telopeptide of type II collagen(CTX-II)
  • 縮寫:
    CTX-Ⅱ
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 86-95  
    1:40 Average % 99  
    Range % 96-102  
    1:80 Average % 92  
    Range % 87-95  
    1:160 Average % 96  
    Range % 93-99  
  • 回收率:
    The recovery of mouse CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-96  
    EDTA plasma (n=4) 94 91-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.290 2.256 2.273 2.112  
    100 1.671 1.666 1.669 1.508  
    50 1.143 1.132 1.138 0.977  
    25 0.718 0.701 0.710 0.549  
    12.5 0.412 0.427 0.420 0.259  
    6.25 0.301 0.285 0.293 0.132  
    3.12 0.228 0.221 0.225 0.064  
    0 0.159 0.163 0.161    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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