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  4. Mouse 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

Mouse 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

  • 中文名稱：
    小鼠6酮前列腺素F1a(6-keto-PGF1a)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09422m
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 pg/mL-1000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠6酮前列腺素F1a(6-keto-PGF1a)酶聯(lián)免疫試劑盒（CSB-E09422m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的6ketoPGF1a含量。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，適用于心血管疾病模型、抗血栓藥物研究、組織缺血再灌注損傷等科研場景的實驗分析，為血管活性物質研究及藥效評價提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    6ketoPGF1a
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 86-109  
    1:2 Average % 92  
    Range % 86-105  
    1:4 Average % 90  
    Range % 85-105  
    1:8 Average % 93  
    Range % 84-100  
  • 回收率：
    The recovery of mouse 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 86-107  
    EDTA plasma (n=4) 94 83-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.130 0.136 0.133    
    500 0.234 0.220 0.227    
    230 0.359 0.343 0.351    
    75 0.618 0.594 0.606    
    25 1.204 1.227 1.216    
    0 2.592 2.625 2.609    
  • 數據處理：
  • 貨期：
    3-5 working days

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