Human secretory immunoglobulin A,SIgA ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人分泌型免疫球蛋白A(SIgA)酶聯(lián)免疫試劑盒（CSB-E09235h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)唾液、尿液、tear樣本中的SIgA含量。SIgA即分泌型免疫球蛋白A，是黏膜免疫的關(guān)鍵效應(yīng)分子。它能在黏膜表面形成保護(hù)層，阻止病原體黏附與入侵。相關(guān)研究聚焦于其產(chǎn)生機(jī)制、與微生物群落互作等，旨在揭示其在抗感染、維持黏膜穩(wěn)態(tài)中的作用，為開(kāi)發(fā)新療法提供依據(jù)。試劑盒檢測(cè)范圍為0.625 μg/ml-40 μg/ml，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中黏膜免疫機(jī)制探索、感染性疾病模型構(gòu)建、營(yíng)養(yǎng)或藥物對(duì)局部免疫影響的評(píng)估等場(chǎng)景，例如通過(guò)檢測(cè)唾液SIgA水平研究口腔免疫應(yīng)答，或分析淚液SIgA濃度變化探索眼表疾病相關(guān)免疫調(diào)節(jié)機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫：
SIgA
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種屬：
Homo sapiens (Human)
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樣本類型：
saliva, urine, tear
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檢測(cè)范圍：
0.625 μg/ml-40 μg/ml.
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靈敏度：
0.312 μg/ml
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測(cè)波長(zhǎng)：
450 nm
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研究領(lǐng)域：
Others
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測(cè)定原理：
quantitative
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測(cè)定方法：
Competitive
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精密度：
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標(biāo)準(zhǔn)曲線：
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
- Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
- One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
- One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
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