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Human phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA kit

  • 中文名稱:
    人磷酸化Tau蛋白酶聯免疫試劑盒
  • 貨號:
    CSB-E17929h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人磷酸化Tau蛋白酶聯免疫試劑盒(CSB-E17929h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、腦脊液(CSF)樣本中的pMAPT /pTAU含量。pMAPT/pTAU作為靶點,在神經退行性疾病如阿爾茨海默病背景下備受關注。它與微管相關蛋白tau異常磷酸化有關。研究機制圍繞探索其磷酸化調控過程、異常磷酸化后對神經細胞功能影響,期望以此為突破點開發疾病治療手段。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,該產品可廣泛應用于神經退行性疾病機制研究、磷酸化Tau蛋白動態變化監測、藥物干預效果評估等科研領域,為探索病理過程中生物標志物變化提供可靠工具,尤其適用于阿爾茨海默病模型研究、體液生物標志物篩查及神經科學相關基礎研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    pMAPT /pTAU
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cerebrospinal fluid (CSF)
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human pMAPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %87
    Range %81-92
    1:4Average %95
    Range %90-99
    1:8Average %101
    Range %96-108
  • 回收率:
    The recovery of human pMAPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9893-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.278 2.184 2.231 2.059
    10001.781 1.725 1.753 1.581
    5001.268 1.211 1.240 1.068
    2500.716 0.742 0.729 0.557
    1250.585 0.537 0.561 0.389
    62.50.369 0.349 0.359 0.187
    31.250.254 0.272 0.263 0.091
    00.174 0.169 0.172  
  • 數據處理:
  • 貨期:
    3-5 working days

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