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Human oxytocin,OT ELISA Kit

  • 中文名稱:
    人催產素(OT)酶聯免疫試劑盒
  • 貨號:
    CSB-E08994h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人催產素(OT)酶聯免疫試劑盒(CSB-E08994h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的OT含量。人催產素(OT)是一種哺乳動物激素,由9個氨基酸構成,主要在大腦下視丘分泌,參與分娩、泌乳和母嬰關系等。其機制研究顯示,OT通過調節神經遞質和受體基因,影響信任、合作和社會認知等行為,具有臨床應用潛力,如治療自閉癥和社交恐懼癥。試劑盒檢測范圍為8 μIU/mL-400 μIU/mL,適用于基礎科研中探索神經生物學機制、行為學關聯性分析或母嬰健康相關研究;該產品為神經科學、心理學及內分泌學領域的研究者提供可靠工具,用于定量分析生物樣本中OT的動態變化,支持探索其在生理調控及疾病模型中的潛在作用。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    OT
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    8 μIU/mL-400 μIU/mL
  • 靈敏度:
    10 μIU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-96  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 98  
    Range % 92-103  
    1:8 Average % 93  
    Range % 87-98  
  • 回收率:
    The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-96  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average Corrected  
    400 2.470 2.498 2.484 2.440  
    160 1.530 1.496 1.513 1.469  
    60 0.649 0.652 0.651 0.607  
    20 0.299 0.313 0.306 0.262  
    8 0.147 0.144 0.146 0.102  
    0 0.045 0.043 0.044    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

I hope you would be able to advice me if this item would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on oxytocin. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
CSB-E08994h is based on double antibody sandwich method. Sorry we are not sure if it is suitable to test human salivary or not. We suggest you do a pretest first. One 96T kit can test 45 samples if you run in duplicates.