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  4. Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

  • 中文名稱：
    人氧化高密度脂蛋白(Ox-HDL)酶聯免疫試劑盒
  • 貨號：
    CSB-E16552h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人氧化高密度脂蛋白(Ox-HDL)酶聯免疫試劑盒（CSB-E16552h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的Ox-HDL含量。Ox - HDL指氧化型高密度脂蛋白，HDL本具抗動脈粥樣硬化等有益作用，但氧化后功能改變。研究機制上，Ox - HDL可通過多種途徑影響細胞功能，比如促使巨噬細胞攝取脂質形成泡沫細胞，參與動脈粥樣硬化等疾病的發生發展。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，可靈活應用于基礎醫學研究領域，如探究氧化應激狀態下脂蛋白代謝機制、評估抗氧化藥物干預效果、建立疾病模型生物標志物分析體系等；滿足細胞實驗、動物模型及臨床前研究中對Ox-HDL的精準定量需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    Ox-HDL
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 80-98  
    1:200 Average % 96  
    Range % 91-100  
    1:400 Average % 94  
    Range % 90-105  
    1:800 Average % 92  
    Range % 86-98  
  • 回收率：
    The recovery of human Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 80-100  
    EDTA plasma (n=4) 90 80-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.813 2.722 2.768 2.607  
    1000 2.122 2.036 2.079 1.918  
    500 1.154 1.198 1.176 1.015  
    250 0.623 0.595 0.609 0.448  
    125 0.368 0.347 0.358 0.197  
    62.5 0.292 0.272 0.282 0.121  
    31.25 0.205 0.192 0.199 0.038  
    0 0.164 0.158 0.161 　  
  • 數據處理：
  • 貨期：
    3-5 working days

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