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Human myelin basic protein(MBP) antibody ELISA Kit

  • 中文名稱:
    人髓鞘堿性蛋白(MBP)抗體酶聯免疫試劑盒
  • 貨號:
    CSB-E04787h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人髓鞘堿性蛋白(MBP)抗體酶聯免疫試劑盒(CSB-E04787h)為雙抗夾心法ELISA試劑盒,定量檢測血清、腦脊液(CSF)樣本中的MBP Ab含量。人髓鞘堿性蛋白(MBP)抗體是一種針對髓鞘堿性蛋白的自身免疫抗體,與多發性硬化癥(MS)等脫髓鞘疾病的發生密切相關。研究顯示,MBP抗體在神經組織細胞膜上裂解MBP,導致髓鞘損傷,進而引發MS。目前,針對MBP抗體的研究主要集中在揭示其與MS等疾病的關系,以及開發針對該靶點的治療策略。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于科研領域中對神經系統疾病模型的機制研究、藥物干預效果評估或生物標志物探索,例如多發性硬化癥、腦創傷或神經退行性疾病的體外實驗分析本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    MBP Ab
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, cerebrospinal fluid (CSF)
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 85-103  
    1:2 Average % 95  
    Range % 84-100  
    1:4 Average % 97  
    Range % 90-105  
    1:8 Average % 92  
    Range % 82-106  
  • 回收率:
    The recovery of human MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 83-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.595 2.658 2.627 2.524  
    20 2.121 2.136 2.129 2.026  
    10 1.467 1.473 1.470 1.367  
    5 0.848 0.896 0.872 0.769  
    2.5 0.475 0.494 0.485 0.382  
    1.25 0.311 0.298 0.305 0.202  
    0.625 0.234 0.253 0.244 0.141  
    0 0.102 0.104 0.103    
  • 數據處理:
  • 貨期:
    3-5 working days

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