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  4. Human melanin ELISA kit

Human melanin ELISA kit

  • 中文名稱：
    人黑色素酶聯免疫試劑盒
  • 貨號：
    CSB-E14051h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人黑色素酶聯免疫試劑盒（CSB-E14051h）為競爭法ELISA試劑盒，定量檢測血清樣本中的melanin含量。Melanin即黑色素，是一種廣泛存在于人和動物體內的生物色素。其在皮膚顏色形成、抵御紫外線等方面發揮重要作用。研究機制上，主要聚焦于其合成途徑、調控因素等，旨在了解相關機制以開發美白、治療色素異常疾病等方面的方法。試劑盒檢測范圍為12.5 pg/mL-1000 pg/mL，適用于皮膚生理機制、色素代謝調控及黑色素相關疾病模型的科學研究；適用于人體血清中黑色素水平的動態監測；可支持黑色素生成調控機制、光老化研究、美白成分篩選等基礎科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    melanin
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum
  • 檢測范圍：
    12.5 pg/mL-1000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-102  
    1:2 Average % 95  
    Range % 91-100  
    1:4 Average % 94  
    Range % 92-97  
    1:8 Average % 95  
    Range % 90-101  
  • 回收率：
    The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-105  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    1000 0.145 0.147 0.146 　  
    375 0.276 0.286 0.281 　  
    187.5 0.402 0.413 0.408 　  
    62.5 0.553 0.562 0.558 　  
    12.5 1.051 1.084 1.068 　  
    0 2.453 2.567 2.510 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Which type of melanin do you dose?The three types have different molecular structures. how can we be sure that the antibodies recognize all the target? And so my question is how can we be sure that we dose the whole melanin? Thanks for your help!

A:
Thanks for your inquiry. Our kit detects the general melanin and has no detailed types. Small molecules are also different in vivo and in vitro. The body may be combined with other proteins or small molecules to aggregate, and our box detects such a total form. In addition, the small molecule is the reference https://pubchem.ncbi.nlm.nih.gov/compound/6325610#section=Top design.Pls let me know if you have any further questions. Thank you.