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Human desmosine(DES) ELISA Kit

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人鎖鏈素酶聯(lián)免疫試劑盒（CSB-E12871h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織勻漿樣本中的DES含量。人鎖鏈素是一種存在于人體結(jié)締組織中的彈性蛋白交聯(lián)氨基酸，負責(zé)彈性蛋白的彈性和黃色特質(zhì)。其研究涉及彈性蛋白的降解與合成，以及與皮膚衰老和疾病的關(guān)系。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可滿足科研領(lǐng)域中對細胞培養(yǎng)模型、動物實驗或臨床前樣本的鎖鏈素水平分析需求，為探索腸道吸收障礙、炎癥性疾病的分子機制或藥物干預(yù)效果評估提供精準(zhǔn)的實驗工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。

縮寫：

DES

種屬：

Homo sapiens (Human)

樣本類型：

serum, urine, tissue homogenates

檢測范圍：

0.156 ng/mL-10 ng/mL

靈敏度：

0.039 ng/mL

反應(yīng)時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領(lǐng)域：

Others

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	92
	Range %	87-98
1:4	Average %	88
	Range %	82-92
1:8	Average %	97
	Range %	94-106

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.932	2.902	2.917	2.827
5	2.173	2.098	2.136	2.046
2.5	1.466	1.421	1.444	1.354
1.25	0.788	0.773	0.781	0.691
0.625	0.462	0.445	0.454	0.364
0.312	0.298	0.279	0.289	0.199
0.156	0.206	0.191	0.199	0.109
0	0.093	0.087	0.090

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DES antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DES antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.