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Human copeptin ELISA kit

  • 中文名稱：
    人和肽素(copeptin)酶聯免疫試劑盒
  • 貨號：
    CSB-E12130h
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人和肽素(copeptin)酶聯免疫試劑盒（CSB-E12130h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養上清液、組織勻漿樣本中的copeptin含量。Copeptin是一種由精氨酸加壓素原羧基末端產生的肽，作為加壓素（抗利尿激素，ADH）的替代物，其水平在多種疾病中升高，如心血管疾病、腎病和肺部疾病等。Copeptin的檢測簡便、快速，可有效地反映內源性AVP的水平，近年來，其在疾病診斷和預后評估方面的作用成為研究熱點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于心血管疾病機制研究、膿毒癥病理模型探索、水鹽代謝相關基礎實驗等領域，支持體外實驗中copeptin水平的動態監測及生物標志物相關性分析，為應激相關疾病的基礎研究提供高效檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    copeptin
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, cell culture supernates, tissue homogenates
  • 檢測范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %82-95
    1:2Average %99
    Range %92-105
    1:4Average %97
    Range %90-106
    1:8Average %96
    Range %91-102
  • 回收率：
    The recovery of human Copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.535 2.752 2.644 2.533
    25002.012 2.179 2.096 1.985
    12501.598 1.599 1.599 1.488
    6250.847 0.867 0.857 0.746
    3120.478 0.502 0.490 0.379
    1560.336 0.356 0.346 0.235
    780.237 0.221 0.229 0.118
    00.109 0.112 0.111 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human copeptin antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled copeptin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

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相關問答

■ 常見問題解答
Q:

Please provide the following information regarding human copeptin ELISA Kit (CSB-E12130h)
Please confirm the concentration of the stock? (5000pg/ml)

A:
Thanks for your inquiry.
The standard in the kit is lyophilized powder. The concentration after reconstitution by sample diluent is 5000pg/ml.Pls let me know if you have any further questions. Thank you.