Human colon cancer-specific antigen-2,CCSA-2 ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人大腸癌專一抗原2(CCSA-2)酶聯免疫試劑盒（CSB-E11291h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CCSA-2含量。試劑盒檢測范圍為3.33 ng/mL-80 ng/mL，適用于大腸癌發病機制研究、抗癌藥物體外篩選、治療效果評估及疾病動物模型中CCSA - 2動態變化分析。適用于實驗室條件下探索CCSA - 2在大腸癌微環境調控、腫瘤侵襲性評價等領域的生物學功能。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CCSA-2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.33 ng/mL-80 ng/mL
靈敏度：

1.33 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	92
	Range %	88-100
1:4	Average %	98
	Range %	92-106
1:8	Average %	95
	Range %	86-101

回收率：

The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.