Human bovine serum albumin (BSA) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

人的牛血清白蛋白(BSA)酶聯(lián)免疫試劑盒（CSB-E08633h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的BSA含量。BSA即牛血清白蛋白，背景上，它來源豐富、價格低廉，在生物醫(yī)學(xué)領(lǐng)域廣泛應(yīng)用。研究機制方面，其可作為載體蛋白，結(jié)合藥物、小分子等；也能模擬體內(nèi)血漿環(huán)境，用于藥物體外研究，還可用于酶活性測定、免疫分析等。試劑盒檢測范圍為0.312 μg/mL-80 μg/mL，靈敏度為0.312 μg/mL。本試劑盒通過預(yù)包被特異性抗體，利用樣本中BSA與酶標記BSA競爭結(jié)合抗體的特性，結(jié)合顯色反應(yīng)實現(xiàn)定量分析，可廣泛用于實驗室評估樣本制備過程中BSA的添加濃度、檢測實驗體系中的外源性BSA干擾，或作為生物制品生產(chǎn)過程中蛋白成分的質(zhì)控工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

BSA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.312 μg/mL-80 μg/mL
靈敏度：

0.312 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of human BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-108
EDTA plasma (n=4)	97	90-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.