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Human beta-crosslaps (bCTx) ELISA kit

  • 中文名稱:
    人β膠原交聯(bCTx)酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027521HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人β膠原交聯(bCTx)酶聯免疫試劑盒(CSB-EQ027521HU)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的bCTx含量。試劑盒檢測范圍為125 pg/mL-2000 pg/mL,在科研場景中,本試劑盒可廣泛應用于骨代謝機制研究、抗骨質疏松藥物開發評估、骨骼疾病動物模型評價等領域,為骨轉換相關的基礎研究提供關鍵數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    bCTx
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    125 pg/mL-2000 pg/mL
  • 靈敏度:
    125 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 104  
    Range % 96-108  
    1:2 Average % 88  
    Range % 83-92  
    1:4 Average % 101  
    Range % 93-104  
    1:8 Average % 106  
    Range % 100-110  
  • 回收率:
    The recovery of human bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 82-97  
    EDTA plasma (n=4) 93 87-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.159 0.155 0.157    
    1000 0.316 0.301 0.309    
    500 0.503 0.492 0.498    
    250 0.919 0.936 0.928    
    125 1.938 1.897 1.918    
  • 數據處理:
  • 貨期:
    3-5 working days

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