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Human anti-growth hormone(GH) antibody ELISA Kit

  • 中文名稱:
    人抗生長激素(GH)抗體酶聯免疫試劑盒
  • 貨號:
    CSB-E09563h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人抗生長激素(GH)抗體酶聯免疫試劑盒(CSB-E09563h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的GH Ab含量。人抗生長激素抗體是一種針對生長激素的特異性抗體,其研究背景涉及生長激素在人體中的作用及其相關疾病的治療。該抗體通過阻斷生長激素的作用,可用于治療因生長激素過量引起的疾病,如肢端肥大癥等。研究機制主要關注其與生長激素受體的相互作用及其在體內的藥理效應。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,該產品適用于基礎醫學研究中對GH相關疾病的機制探索、藥物干預效果評估或內分泌調控研究,例如在動物模型或體外實驗中分析抗體與GH的相互作用,或評估特定病理狀態下GH抗體表達動態變化,為科研人員提供可靠的實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    GH Ab
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    7.8 pg/mL-500 pg/mL
  • 靈敏度:
    2 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Developmental Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human anti-GH antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 82-105  
    1:2 Average % 94  
    Range % 84-105  
    1:4 Average % 97  
    Range % 92-101  
    1:8 Average % 95  
    Range % 82-105  
  • 回收率:
    The recovery of human anti-GH antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 82-105  
    EDTA plasma (n=4) 93 84-102  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.604 2.573 2.589 2.448  
    250 1.994 2.109 2.052 1.911  
    125 1.382 1.361 1.372 1.231  
    62.5 0.852 0.845 0.849 0.708  
    31.2 0.524 0.516 0.520 0.379  
    15.6 0.356 0.368 0.362 0.221  
    7.8 0.242 0.253 0.248 0.107  
    0 0.145 0.136 0.141    
  • 數據處理:
  • 貨期:
    3-5 working days

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