Human acylation stimulating protein(ASP) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人促?；鞍?ASP)酶聯(lián)免疫試劑盒（CSB-E13474h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ASP含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于探索肥胖癥、2型糖尿病、代謝綜合征等疾病的分子機(jī)制研究，也可用于體外實(shí)驗(yàn)?zāi)Ｐ椭兄敬x相關(guān)藥物或營養(yǎng)干預(yù)的效果評估，為代謝領(lǐng)域基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

ASP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	91-103
1:200	Average %	89
	Range %	84-95
1:400	Average %	95
	Range %	90-99
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	90	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.