Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人酒石酸鹽抵抗的酸性磷酸酶(TRAP)酶聯免疫試劑盒（CSB-E13415h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TRAP含量。試劑盒檢測范圍為0.47 ng/mL-30 ng/mL，適用于體外骨代謝研究、破骨細胞活性評估、骨相關疾病機制探索以及藥物干預效果評價等科研領域，其高靈敏度的檢測性能可有效支持微量樣本中TRAP的動態變化分析，為骨生物學、細胞信號通路等基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

TRAP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.47 ng/mL-30 ng/mL
靈敏度：

0.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	97
	Range %	95-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of human TRAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	89	84-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.