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Human Substance P,SP ELISA Kit

  • 中文名稱:
    人p物質(SP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08357h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人p物質(SP)酶聯免疫試劑盒(CSB-E08357h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SP含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL,適用于神經生物學、免疫調節機制等基礎研究,特別在疼痛模型評估、神經炎癥研究或胃腸道功能實驗中,可為探索SP在病理生理過程中的動態變化提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    SP
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    6.25 pg/mL-400 pg/mL
  • 靈敏度:
    1.56 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %92-100
    1:2Average %102
    Range %98-105
    1:4Average %89
    Range %85-96
    1:8Average %104
    Range %100-108
  • 回收率:
    The recovery of human SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8682-92
    EDTA plasma (n=4)8581-90
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.439 2.489 2.464 2.290
    2001.933 1.978 1.956 1.782
    1001.285 1.298 1.292 1.118
    500.799 0.782 0.791 0.617
    250.475 0.468 0.472 0.298
    12.50.376 0.394 0.385 0.211
    6.250.316 0.308 0.312 0.138
    00.175 0.173 0.174  
  • 數據處理:
  • 貨期:
    3-5 working days

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