Human Prostaglandin F2α,PGF2α ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人前列腺素F2α(PGF2α)酶聯免疫試劑盒（CSB-E10142h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PGF2α含量。PGF2α 是重要靶點。它作為前列腺素家族一員，在人體生理過程如生殖、心血管調節等方面發揮作用。研究機制上，其通過與特定受體結合，激活下游信號通路，影響細胞功能和生理反應，在相關疾病治療領域具潛在研究價值。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，該產品適用于探索PGF2α在生殖系統模型（如前列腺功能研究）、心血管疾病機制、炎癥反應調控及藥物干預實驗中的生物學作用，為細胞信號通路研究、疾病分子機制解析及潛在生物標志物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PGF2 alpha,Prostaglandin F2 alpha
縮寫：

PGF2α
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	102
	Range %	97-107
1:4	Average %	93
	Range %	85-99
1:8	Average %	98
	Range %	91-104

回收率：

The recovery of human PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	88	81-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.