Human Pepsinogen I ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人胃蛋白酶原I酶聯(lián)免疫試劑盒（CSB-E17538h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PG I含量。PG I（胃蛋白酶原I）是胃中的一種消化酶，其生物學(xué)功能包括消化食物中的蛋白質(zhì)。研究顯示，PG I在心血管系統(tǒng)中具有重要作用，其合成能力由內(nèi)向外依次減弱，以內(nèi)皮細(xì)胞合成能力最強。PG I的研究機制涉及其對心血管系統(tǒng)的影響，以及其在胃癌診斷中的應(yīng)用。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，支持科研人員高效開展胃功能研究、胃黏膜損傷機制探索或藥物干預(yù)效果評估等實驗。其寬泛的線性范圍和多類型樣本兼容性，特別適用于縱向研究或不同實驗條件間的數(shù)據(jù)比對，為胃相關(guān)疾病的分子機制解析及生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PG I
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	106
	Range %	100-115
1:4	Average %	97
	Range %	94-112
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.