Human Pepsin,PG ELISA Kit

產品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人胃蛋白酶(PG)酶聯免疫試劑盒（CSB-E08919h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的PG含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于消化系統疾病機制研究、胃功能相關生物標志物探索、營養代謝評估等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PGA3 ELISA Kit; Pepsin A-3 ELISA Kit; EC 3.4.23.1 ELISA Kit; Pepsinogen-3 ELISA Kit
縮寫：

PG
Uniprot No.：

P0DJD8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	84-95
1:200	Average %	93
	Range %	88-99
1:400	Average %	99
	Range %	95-104
1:800	Average %	101
	Range %	96-108

回收率：

The recovery of human PG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-104
EDTA plasma (n=4)	94	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
基因功能參考文獻：
1. Low Pepsinogen I is associated with precancerous gastric lesions. PMID: 25292040
2. High serum Pepsinogen I level is associ9ated with the progression of gastric precancerous lesions. PMID: 24895149
3. Significantly higher AUC was observed for the PGI/PGII ratio and is associated with atrophy and intestinal metaplasia. PMID: 23886209
4. A significant negative correlation was found between the degree of corpus atrophy and the level of serum pepsinogen-I in atrophic gastritis patients with gastroesophageal reflux. PMID: 21175799
5. Higher levels of Pepsinogen I is associated with early gastric cancer PMID: 15688378
6. Helicobacter pylori eradication improves gastric histology and decreases serum gastrin, pepsinogen I and pepsinogen II levels in patients with duodenal ulcer. PMID: 17559360
7. pepsinogens may have a role in esophageal squamous dysplasia PMID: 18844222
8. Barrett's esophagus is characterized by the absence of Helicobacter pylori infection and high levels of serum pepsinogen I concentration in Japan. PMID: 19196398
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亞細胞定位：

Secreted.
蛋白家族：

Peptidase A1 family
數據庫鏈接：

HGNC: 8885

OMIM: 169700

KEGG: hsa:643834

STRING: 9606.ENSP00000322192

UniGene: Hs.601055