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Human Pentosidine ELISA Kit

  • 中文名稱:
    人戊糖素(Pentosidine)酶聯免疫試劑盒
  • 貨號:
    CSB-E09415h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人戊糖素(Pentosidine)酶聯免疫試劑盒(CSB-E09415h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、尿液樣本中的Pentosidine含量。Pentosidine是一個靶點,它屬于晚期糖基化終末產物(AGEs)。在體內,它主要通過美拉德反應形成并逐漸積累。研究機制方面,它與糖尿病并發癥、衰老等密切相關,可引發氧化應激和炎癥反應,常作為評估相關疾病風險和進程的生物標志物。試劑盒檢測范圍為31.25 pmol/mL-2000 pmol/mL,該產品適用于基礎醫學研究、疾病機制探索、藥物干預效果評估等科研場景,為代謝性疾病、衰老相關疾病及骨關節健康研究提供可靠的生物標志物檢測方案。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    Pentosidine
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, urine
  • 檢測范圍:
    31.25 pmol/mL-2000 pmol/mL
  • 靈敏度:
    7.81 pmol/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %94
    Range %81-102
    1:2Average %95
    Range %84-105
    1:4Average %104
    Range %91-111
    1:8Average %95
    Range %80-103
  • 回收率:
    The recovery of human Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-105
    EDTA plasma (n=4)9488-105
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    20002.677 2.697 2.687 2.537
    10001.843 1.864 1.854 1.704
    5001.163 1.153 1.158 1.008
    2500.742 0.754 0.748 0.598
    1250.447 0.461 0.454 0.304
    62.50.347 0.359 0.353 0.203
    31.250.268 0.262 0.265 0.115
    00.154 0.146 0.150  
  • 數據處理:
  • 貨期:
    3-5 working days

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