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Human POTE ankyrin domain family member E(POTEE) ELISA kit

  • 中文名稱:
    人POTE錨蛋白域家庭成員E(POTEE)酶聯免疫試劑盒
  • 貨號:
    CSB-EL740863HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人POTE錨蛋白域家庭成員E(POTEE)酶聯免疫試劑盒(CSB-EL740863HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的POTEE含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,適用于基礎醫學研究中POTEE蛋白的定量檢測,可用于探索其在腫瘤微環境中的功能機制、評估不同病理狀態下蛋白表達水平變化,或作為體外模型研究中細胞信號通路調控的檢測工具,為腫瘤生物學、分子病理學等領域的科研工作提供標準化檢測方案。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    POTEE ELISA Kit; A26C1A ELISA Kit; POTE2 ELISA Kit; POTE ankyrin domain family member E ELISA Kit; ANKRD26-like family C member 1A ELISA Kit; Prostate ELISA Kit; ovary ELISA Kit; testis-expressed protein on chromosome 2 ELISA Kit; POTE-2 ELISA Kit
  • 縮寫:
    POTEE
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human POTEE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 98
    Range % 93-103
    1:4 Average % 102
    Range % 98-106
    1:8 Average % 91
    Range % 86-97
  • 回收率:
    The recovery of human POTEE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 96-108
    EDTA plasma (n=4) 89 83-94
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.786 2.934 2.860 2.729
    800 2.214 2.341 2.278 2.147
    400 1.425 1.467 1.446 1.315
    200 0.743 0.754 0.749 0.618
    100 0.456 0.446 0.451 0.320
    50 0.327 0.312 0.320 0.189
    25 0.216 0.211 0.214 0.083
    0 0.132 0.130 0.131  
  • 數據處理:
  • 貨期:
    3-5 working days

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