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  4. Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

  • 中文名稱：
    人骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒
  • 貨號：
    CSB-E05128h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/ml-2000 pg/ml
  • 靈敏度：
    7.8 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒（CSB-E05128h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BGLAP含量。BGLAP（骨鈣素）是一種由成骨細胞分泌的激素，參與骨骼重塑和能量代謝。其羧化形式負向調節骨形成，同時促進骨吸收和礦化，對磷灰石和鈣有強親和力。研究顯示，BGLAP在骨骼發育、成骨細胞分化和生物礦化組織中發揮重要作用。試劑盒檢測范圍為31.25 pg/ml-2000 pg/ml，靈敏度為7.8 pg/ml。適用于骨代謝相關基礎研究，如骨質疏松癥動物模型評估、骨骼發育機制探索、骨組織工程材料生物活性分析等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    98

    Range %

    95-106

    1:2

    Average %

    99

    Range %

    93-105

    1:4

    Average %

    94

    Range %

    86-99

    1:8

    Average %

    97

    Range %

    91-105

  • 回收率：

    The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    99

    96-103

    EDTA plasma (n=4)

    97

    94-101

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.552

    2.634

    2.593

    2.483

    1000

    1.764

    1.667

    1.716

    1.605

    500

    1.242

    1.228

    1.235

    1.125

    250

    0.747

    0.729

    0.738

    0.628

    125

    0.476

    0.451

    0.463

    0.353

    62.5

    0.324

    0.333

    0.329

    0.218

    31.25

    0.194

    0.184

    0.189

    0.079

    0

    0.111

    0.109

    0.110

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human BGLAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled BGLAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展：
        BGLAP (Bone Gla Protein or Osteocalcin) 是一種在骨細胞中高度表達的蛋白質，參與骨代謝和鈣離子調節。近期的研究表明，BGLAP 可能也參與神經系統的調節和認知功能。BGLAP 被認為是一個骨特異性蛋白質，它參與了骨形成和骨質疏松癥的發病機制。BGLAP 的水平可用于評估骨轉換和疾病的進展。
     
  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 基因功能參考文獻：
    1. The results of this study suggest that serum osteocalcin is associated with the onset of puberty in girls. PMID: 30089739
    2. The correlation between Mycobacterium avium subsp. paratuberculosis (MAP) infection and elevation of sera ucOC, reduction of active osteocalcin and increased calcium supports MAP infection role in Crohn's disease and complications with osteoporosis. PMID: 29638140
    3. The results showed no significant correlation between osteocalcin HindIII gene polymorphism and bone mineral density (BMD) and therefore osteocalcin HindIII gene polymorphism may not have major effects on BMD variation in postmenopausal North Indian women. PMID: 30179426
    4. Decreased circulating carboxylated osteocalcin is associated with low-energy bone fractures in children. PMID: 29882816
    5. a decrease in serum osteocalcin levels is associated with future development of type 2 diabetes mellitus. PMID: 28766135
    6. Findings from the available data indicated an overall significant inverse association between serum osteocalcin and body mass index--{REVIEW} PMID: 28822067
    7. relatively lower serum osteocalcin levels were associated with a higher risk of MACE in Chinese men with CAD PMID: 28921598
    8. undercarboxylated form of osteocalcin in serum not related significantly to semen parameters or measured hormones in male infertility PMID: 28000970
    9. serum level not correlated with gender, race/ethnicity, age, diabetes type, BMI percentile, random C-peptide, glucose, or HbA1c at pediatric diabetes diagnosis; serum level inversely correlated with HbA1c at first office visit following diagnosis PMID: 28093839
    10. Serum osteocalcin was directly and independently correlated with circulating uric acid in a population of healthy obese subjects. PMID: 28847266
    11. gamma-carboxylation of OCN on Glu17 residue correlates with insulin resistance and subclinical inflammation, suggesting that gamma-carboxylation of OCN negatively regulates its endocrine action PMID: 28242268
    12. There were differences in the associations between total osteocalcin and glucose metabolism in Han and Uygur T2DM patients, indicating genetic factors may play a role in modulating OC and glucose metabolism in different ethnic population. PMID: 28732499
    13. The study demonstrated that undercarboxylated osteocalcin (ucOC) is related to 25(OH)D and adiponectin concentrations. Both ucOC and 25(OH)D were lower in overweight and obese adolescents. PMID: 28443718
    14. Increased advanced glycation end products and protein oxidative damage markers in the course of obesity may contribute to decreased carboxylated osteocalcin serum level. PMID: 28841723
    15. Progressive calcification of atherosclerotic plaques is accompanied by insignificant accumulation of calcitonin and osteoprotegerin, whereas the osteocalcin expression significantly increased during calcification. PMID: 28429221
    16. Undercarboxylated osteocalcin reflects the reserve capacity of beta-cell function, such as the bolus insulin secretion ability in patients with type 2 diabetes. PMID: 27889949
    17. XPS showed that similar amounts of hOC and dhOC were absorbed onto hydroxyapaptite and octacalcium phosphate surfaces, but ToF-SIMS detected some small differences in the amino acid fragment intensities on these surfaces for adsorbed hOC and dhOC. PMID: 28521505
    18. Common polymorphism rs1800247 in osteocalcin gene was associated with hypertension and diastolic blood pressure levels. The association of rs1800247 with hypertension was affected by its interaction with HOMA-IR. PMID: 27251081
    19. These findings indicate the undercarboxylated form of OC is not implicated in age-related changes in skeletal muscle or adipose tissue mass in older community-dwelling adults. PMID: 27604070
    20. Demonstrate a positive association between osteocalcin, a factor known to regulate bone remodelling, with cognitive performance in older non-demented women. PMID: 27515675
    21. Common genetic variants of the osteocalcin gene may not be a major contributor to variations in serum osteocalcin or BMD in postmenopausal and elderly Chinese women. PMID: 28002816
    22. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. PMID: 28922389
    23. Low serum osteocalcin concentrations are associated with type 2 diabetes. PMID: 27061949
    24. Decreased blood concentration of Carboxylated osteocalcin is associated with metabolic complications and inflammation. PMID: 27667744
    25. Total and undercarboxylated osteocalcin levels were significantly correlated with insulin secretion and insulin resistance indices, but not adiponectin levels, in pregnant women. PMID: 26708046
    26. Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID: 27673554
    27. Osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of mesenchymal stromal cells (MSCs). PMID: 28106724
    28. Serum osteocalcin levels in pediatric obstructive sleep apnea hypopnea syndrome patients were significantly lower than normal controls before adenotonsillectomy. PMID: 26938749
    29. the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients, was examined. PMID: 26743527
    30. Reduced plasma Osteocalcin levels are associated with cardiovascular and metabolic diseases. PMID: 26863345
    31. An overview of osteocalcin progress. PMID: 26747614
    32. the BMP-2/Smad1/5/RUNX2 signaling pathway participates in the silicon-mediated induction of COL-1 and osteocalcin synth PMID: 27025722
    33. Data indicate that lower osteocalcin was an independent predictor of worse cognitive performance on multivariate analysis. PMID: 26406918
    34. A common polymorphism rs1800247 in osteocalcin gene may effect the risk of osteoporosis and fracture and serum osteocalcin levels in Chinese. PMID: 26194493
    35. There is an inverse relationship between the visfatin and undercarboxylated osteocalcin in type 2 diabetes patients with diabetic kidney disease. PMID: 26922969
    36. This study investigated osteocalcin, metabolic parameters and anthropometric characteristics in normal weight and overweight/obese children. PMID: 26772621
    37. Nonalcoholic fatty liver disease negatively associated with right-hip bone mineral density and serum osteocalcin in Korean men. PMID: 26636404
    38. The aims of this study were to evaluate OCN and sclerostin levels in subjects who underwent coronary artery bypass graft (CABG) surgery compared with those in normal controls. PMID: 26576474
    39. serum level not associated with weight loss or body fat percentage PMID: 25963022
    40. Serum osteocalcin levels are inversely correlated with nonalcoholic fatty liver disease in postmenopausal Chinese women with normal blood glucose levels. PMID: 26567728
    41. Serum total osteocalcin level is significantly lower in 2 diabetes mellitus patients than controls. PMID: 26372899
    42. Elevated serum preptin and decreased osteocalcin concentrations, together with insulin resistance, are associated with obesity and overweight. PMID: 25675353
    43. gestational diabetes is not associated with osteocalcin, under-carboxylated osteocalin, osteopontin and leptin and does not correlate with insulin resistance. PMID: 25480426
    44. Serum uOC levels in non-dialysis patients with CKD are significantly lower than those in healthy individuals, and uOC is closely associated with subclinical atherosclerosis in CKD patients. PMID: 26381729
    45. Endocrine function of osteocalcin and the cell biology events that allow osteocalcin to become a hormone. [review] PMID: 25577163
    46. Data indicate that higher osteocalcin (OC) or its undercarboxylated form (ucOC) concentration and ucOC to osteocalcin ratio (OCR) were associated with the risk of coronary calcification in men. PMID: 25868823
    47. Data suggest that a low serum osteocalcin level was an independent risk factor for abdominal obesity. PMID: 25871789
    48. Correlation between osteocalcin-positive endothelial progenitor cells and spotty calcification in patients with coronary artery disease PMID: 25644945
    49. These findings unequivocally document that increased vitamin K intake reduces the uncarboxylated form of OCN PMID: 25855755
    50. Serum osteocalcin level was not independently associated with C-IMT in a metabolically healthy Chinese population. PMID: 26077201

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 數據庫鏈接：

    HGNC: 1043

    OMIM: 112260

    KEGG: hsa:632

    STRING: 9606.ENSP00000357255

    UniGene: Hs.654541