Human Orexin B,OX-B ELISA Kit

說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

人食欲素/阿立新B(OX-B)酶聯(lián)免疫試劑盒（CSB-E09863h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的OX-B含量。人食欲素/阿立新B（OX-B）是一種由下丘腦神經(jīng)元分泌的神經(jīng)肽，能促進(jìn)食欲、調(diào)節(jié)睡眠和覺醒。研究發(fā)現(xiàn)，OX-B與食欲素受體結(jié)合后，可調(diào)節(jié)攝食行為、睡眠周期及情緒等生理過程。針對(duì)OX-B及其受體的研究，為治療肥胖、失眠等疾病提供了新的治療靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于神經(jīng)生物學(xué)研究中食欲素系統(tǒng)的功能解析、代謝調(diào)節(jié)機(jī)制探索，或睡眠障礙動(dòng)物模型中的生物標(biāo)志物分析，為中樞神經(jīng)系統(tǒng)調(diào)控研究提供可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

OX-B
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	95-111
1:2	Average %	100
	Range %	93-104
1:4	Average %	94
	Range %	86-97
1:8	Average %	100
	Range %	92-104

回收率：

The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	108	97-112

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.