Human L-Carnitine ELISA Kit

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Human L-Carnitine ELISA Kit

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中文名稱：

人左旋肉堿/L-肉堿酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E13606h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

人左旋肉堿/L-肉堿酶聯(lián)免疫試劑盒（CSB-E13606h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液、組織勻漿樣本中的L-Carnitine含量。L-Carnitine即左旋肉堿，它是一種促使脂肪轉(zhuǎn)化為能量的類氨基酸，存在于人體中。在研究機(jī)制方面，它能將長(zhǎng)鏈脂肪酸轉(zhuǎn)運(yùn)到線粒體中進(jìn)行氧化，從而促進(jìn)脂肪代謝。臨床上還可用于輔助治療一些疾病，在減肥等領(lǐng)域也備受關(guān)注。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于代謝調(diào)控機(jī)制研究、運(yùn)動(dòng)醫(yī)學(xué)評(píng)估、營(yíng)養(yǎng)代謝實(shí)驗(yàn)及藥物開發(fā)中的生物標(biāo)志物檢測(cè)；滿足實(shí)驗(yàn)室對(duì)脂肪酸代謝、心肌能量代謝及線粒體功能相關(guān)研究的精準(zhǔn)定量需求；為探索L - 肉堿在能量代謝異常、肥胖癥或運(yùn)動(dòng)恢復(fù)等領(lǐng)域的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

L-Carnitine
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Biochemicals
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	88
	Range %	80-96
1:400	Average %	97
	Range %	91-105
1:800	Average %	99
	Range %	92-110
1:1600	Average %	100
	Range %	90-110

回收率：

The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-110
EDTA plasma (n=4)	97	90-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
20	2.749	2.639	2.694	2.543
10	2.010	1.976	1.993	1.842
5	1.303	1.294	1.299	1.148
2.5	0.889	0.839	0.864	0.713
1.25	0.561	0.524	0.543	0.392
0.625	0.371	0.359	0.365	0.214
0.312	0.256	0.239	0.248	0.097
0	0.155	0.147	0.151

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The correlation between carbamazepine and valproic acid monotherapy with serum adiponectin and carnitine F ?im?ek,Acta Neurol Belg,2020
Effects of L-carnitine supplementation on lipid profiles in patients with coronary artery disease. Lee BJ.et al,Lipids Health Dis.,2016
Carnitine deficiency and its possible risk factors in TB patients: first report Hatamkhani S et al,Immunotherapy,2013
Allopurinol, quercetin and rutin ameliorate renal NLRP3 inflammasome activation and lipid accumulation in fructose-fed rats /,Biochemical Pharmacology,2012

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.