Human Isodesmosine (IDES) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人異鎖鏈素(IDES)酶聯(lián)免疫試劑盒（CSB-E14052h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的IDES含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，科研人員可利用探索IDES在器官纖維化模型中的表達(dá)特征，分析其在炎癥性疾病動(dòng)物或細(xì)胞實(shí)驗(yàn)中的動(dòng)態(tài)變化，或通過檢測體液樣本評估其作為生物標(biāo)志物的潛在價(jià)值，為疾病機(jī)制研究提供可靠工具。適用于體外研究場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IDES
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IDES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	90-99
1:4	Average %	99
	Range %	94-103
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of human IDES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	99	94-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.