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Human Interferon α,IFN-α ELISA Kit

  • 中文名稱:
    人α干擾素(IFN-α)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08636h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人α干擾素(IFN-α)酶聯(lián)免疫試劑盒(CSB-E08636h)為雙抗夾心法ELISA試劑盒,定量檢測樣本中的IFN-α含量。IFN-α即α-干擾素,是一類具有多種生物活性的細胞因子。背景上,它在抗病毒、抗腫瘤及免疫調(diào)節(jié)等方面有重要作用。研究機制方面,它能與細胞表面受體結(jié)合,激活相關(guān)信號通路,誘導(dǎo)多種抗病毒蛋白產(chǎn)生,抑制病毒復(fù)制,還可調(diào)節(jié)免疫細胞功能。科研人員可通過精準分析病毒感染模型中的免疫激活狀態(tài)、評估免疫調(diào)節(jié)藥物對IFN-α分泌的影響,或探究腫瘤免疫治療過程中的細胞因子動態(tài)變化。為免疫機制研究及生物制劑開發(fā)提供高效檢測方案。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    IFL ELISA Kit; IFN alpha ELISA Kit; IFN alpha 1/13 ELISA Kit; IFN alpha 1b ELISA Kit; IFN alphaD ELISA Kit; IFN ELISA Kit; IFN-alpha-1/13 ELISA Kit; IFNA@ ELISA Kit; IFNA1 ELISA Kit; IFNA1_HUMAN ELISA Kit; IFNA13 ELISA Kit; Interferon alpha 1 ELISA Kit; Interferon alpha-1/13 ELISA Kit; Interferon alpha-D ELISA Kit; Interferon alpha1 ELISA Kit; Interferon, alpha 13 ELISA Kit; LeIF D ELISA Kit
  • 縮寫:
    IFN-α
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 檢測范圍:
    7.8 pg/ml-500 pg/ml
  • 靈敏度:
    1.95 pg/ml
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

  • 線性度:

  • 回收率:

  • 標準曲線:

  • 本試劑盒所含材料:
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IFN-αantibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IFN-α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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