Human Immunoglobulin M,IgM ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒（CSB-E07976h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的IgM含量。試劑盒檢測范圍為4.68 ng/mL-300 ng/mL，靈敏度為1.17 ng/mL。該產(chǎn)品適用于免疫學基礎(chǔ)研究、感染性疾病模型建立、疫苗免疫效果評估等科研場景，可定量分析體液或組織樣本中的IgM水平變化，為炎癥反應(yīng)、B細胞功能研究及免疫相關(guān)疾病機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

IgM
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

4.68 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	86
	Range %	80-94
1:10000	Average %	97
	Range %	91-105
1:20000	Average %	95
	Range %	91-102
1:40000	Average %	94
	Range %	86-101

回收率：

The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.