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  • 中文名稱:
    人透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E04805h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒(CSB-E04805h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、腦脊液(CSF)、組織勻漿樣本中的HA含量。HA即血凝素,是流感病毒表面重要糖蛋白。其背景上,它在病毒感染宿主過(guò)程中至關(guān)重要。研究機(jī)制方面,HA可介導(dǎo)病毒與宿主細(xì)胞結(jié)合及膜融合,促使病毒侵入細(xì)胞。對(duì)HA研究有助于研發(fā)流感疫苗、抗病毒藥物等,以應(yīng)對(duì)流感威脅。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,科研領(lǐng)域可應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究中HA代謝機(jī)制探索、藥物干預(yù)效果評(píng)價(jià),或結(jié)合動(dòng)物模型、細(xì)胞實(shí)驗(yàn)進(jìn)行跨物種、多組織類型的HA動(dòng)態(tài)監(jiān)測(cè),為組織再生、炎癥調(diào)控及疾病機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    N/A
  • 縮寫:
    HA
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 檢測(cè)范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 87  
    Range % 80-94  
    1:40 Average % 92  
    Range % 88-99  
    1:80 Average % 97  
    Range % 91-100  
    1:160 Average % 101  
    Range % 97-105  
  • 回收率:
    The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-105  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.727 2.700 2.714 2.626  
    5 2.231 2.345 2.288 2.200  
    2.5 1.558 1.655 1.607 1.519  
    1.25 0.831 0.901 0.866 0.778  
    0.625 0.477 0.451 0.464 0.376  
    0.312 0.301 0.322 0.312 0.224  
    0.156 0.189 0.174 0.182 0.094  
    0 0.087 0.089 0.088    
  • 本試劑盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型:細(xì)胞培養(yǎng)上清液

樣品信息:人

稀釋比:1:100

產(chǎn)品評(píng)價(jià): 我用CSB-E04805h檢測(cè)血清樣本中HA含量,試劑盒靈敏度高,數(shù)據(jù)理想。

By 張老師

樣品類型:血漿(抗凝劑) edta

樣品信息:人

稀釋比:1:100

產(chǎn)品評(píng)價(jià): 我們用CSB-E04805h檢測(cè)人冠心病患者血漿臨床樣本,檢測(cè)后的標(biāo)曲擬合結(jié)果達(dá)到0.999,復(fù)孔的CV%變異系數(shù)<10,效果較好,使用方便,為實(shí)驗(yàn)提供了有力的支持

By 類老師

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

A:
Thanks for your inquiry.
CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
We think it can not be detected out by the kit.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    HA(透明質(zhì)酸)是一種大分子多糖,廣泛存在于人體組織中。最近的研究表明,HA在細(xì)胞間通訊、細(xì)胞遷移和組織再生中發(fā)揮重要作用。HA在軟骨組織再生和修復(fù)中的應(yīng)用也受到廣泛關(guān)注。研究人員正在開(kāi)發(fā)一些基于HA的藥物和材料,用于治療各種軟骨損傷和疾病,如骨關(guān)節(jié)炎、肩周炎和韌帶損傷等。


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