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  4. Human Glycogen phosphorylase BB,GP-BB ELISA Kit

Human Glycogen phosphorylase BB,GP-BB ELISA Kit

  • 中文名稱：
    人糖原磷酸化酶同工酶BB(GP-BB)酶聯免疫試劑盒
  • 貨號：
    CSB-E08475h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人糖原磷酸化酶同工酶BB(GP-BB)酶聯免疫試劑盒（CSB-E08475h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GP-BB含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，本產品適用于科研領域中心肌損傷相關機制研究。該產品可用于心肌缺血動物模型評估、心臟疾病機制研究以及藥物干預效果分析等科研場景，為體外實驗提供定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    GP-BB
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GP-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %85-98
    1:2Average %96
    Range %90-102
    1:4Average %95
    Range %90-101
    1:8Average %95
    Range %84-102
  • 回收率：
    The recovery of human GP-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-100
    EDTA plasma (n=4)9692-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    403.047 3.108 3.078 2.901
    202.475 2.557 2.516 2.339
    101.839 1.785 1.812 1.635
    51.128 1.124 1.126 0.949
    2.50.724 0.719 0.722 0.545
    1.250.496 0.468 0.482 0.305
    0.6250.330 0.332 0.331 0.154
    00.177 0.176 0.177 　
  • 數據處理：
  • 貨期：
    3-5 working days

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