Human Free Testosterone,F-TESTO ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (8) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人游離睪酮(F-TESTO)酶聯(lián)免疫試劑盒（CSB-E05096h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的F-TESTO含量。人游離睪酮（F-TESTO）是睪酮的一種游離形式，對(duì)男性健康和生理功能至關(guān)重要。其研究背景涉及睪酮的生理作用、代謝途徑及其與疾病如前列腺癌的關(guān)系。研究機(jī)制主要關(guān)注F-TESTO在體內(nèi)的生物活性、代謝過(guò)程及其對(duì)健康的影響。試劑盒檢測(cè)范圍為7.5 pg/mL-1500 pg/mL，適用于精準(zhǔn)分析低濃度樣本。適用于科研場(chǎng)景下探究?jī)?nèi)分泌失調(diào)、多囊卵巢綜合征、代謝綜合征等疾病的分子機(jī)制，或評(píng)估運(yùn)動(dòng)生理學(xué)、衰老相關(guān)激素水平變化等研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

F-TESTO
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測(cè)范圍：

7.5 pg/mL-1500 pg/mL
靈敏度：

3.75 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	105	101-109