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  4. Human Fibrinogen(FB) ELISA Kit

Human Fibrinogen(FB) ELISA Kit

  • 中文名稱：
    人纖維蛋白原（Fb）酶聯免疫試劑盒
  • 貨號：
    CSB-E13656h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人纖維蛋白原（Fb）酶聯免疫試劑盒（CSB-E13656h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Fb含量。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，該產品適用于基礎醫學研究中凝血/纖溶系統調控機制探索、肝臟功能評估、心血管疾病動物模型驗證，以及炎癥相關病理過程的分子機制研究，為體外藥物篩選、疾病模型構建和生物標志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    Fb
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 μg/mL-20 μg/mL
  • 靈敏度：
    0.078 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 85  
    Range % 80-92  
    1:200 Average % 98  
    Range % 90-105  
    1:400 Average % 97  
    Range % 90-105  
    1:800 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-98  
    EDTA plasma (n=4) 98 90-106  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected  
    20 2.648 2.701 2.675 2.583  
    10 2.159 2.169 2.164 2.072  
    5 1.476 1.536 1.506 1.414  
    2.5 0.864 0.876 0.870 0.778  
    1.25 0.443 0.475 0.459 0.367  
    0.625 0.234 0.253 0.244 0.152  
    0.312 0.146 0.154 0.150 0.058  
    0 0.095 0.089 0.092 　  
  • 數據處理：
  • 貨期：
    3-5 working days

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