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Human Dihydrotestosterone,DHT ELISA Kit

  • 中文名稱:
    人雙氫睪酮(DHT)酶聯免疫試劑盒
  • 貨號:
    CSB-E07878h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人雙氫睪酮(DHT)酶聯免疫試劑盒(CSB-E07878h)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的DHT含量。DHT即雙氫睪酮,是睪酮經5α-還原酶作用轉化而來。它與雄激素性脫發、前列腺增生等密切相關。研究機制主要聚焦于其與雄激素受體結合,影響毛囊、前列腺等組織細胞的生長、分化,為相關疾病的藥物研發提供方向。試劑盒檢測范圍為10 pg/mL-2000 pg/mL,本產品可廣泛應用于激素作用機制研究、內分泌系統功能評估、前列腺相關疾病模型構建及藥物干預效果分析等科研領域,為探索DHT在生理病理過程中的調控網絡提供高效工具,適用于細胞實驗、動物模型及臨床前研究的數據獲取。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    DHT
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    10 pg/mL-2000 pg/mL
  • 靈敏度:
    5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-97  
    1:2 Average % 89  
    Range % 85-99  
    1:4 Average % 93  
    Range % 86-105  
    1:8 Average % 94  
    Range % 87-103  
  • 回收率:
    The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 97 92-105  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.179 0.185 0.182    
    500 0.313 0.319 0.316    
    160 0.523 0.529 0.526    
    40 0.818 0.802 0.810    
    10 1.317 1.330 1.324    
    0 1.952 1.943 1.948    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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