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  4. Human Complement fragment 3c,C3c ELISA Kit

Human Complement fragment 3c,C3c ELISA Kit

  • 中文名稱：
    人補(bǔ)體片段3c(C3c)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11168h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人補(bǔ)體片段3c(C3c)酶聯(lián)免疫試劑盒（CSB-E11168h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液、唾液樣本中的C3c含量。C3c是補(bǔ)體系統(tǒng)中的一個(gè)重要成分，參與經(jīng)典和替代途徑的補(bǔ)體激活。其在腫瘤免疫和病毒感染中發(fā)揮重要作用，如通過(guò)調(diào)節(jié)免疫反應(yīng)和直接殺傷腫瘤細(xì)胞來(lái)抑制腫瘤生長(zhǎng)，同時(shí)在HBV宮內(nèi)傳播中與免疫復(fù)合物相互作用。C3c的研究有助于深入理解補(bǔ)體系統(tǒng)在免疫調(diào)節(jié)和疾病發(fā)生中的作用。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域中對(duì)補(bǔ)體系統(tǒng)功能的探索，例如在炎癥機(jī)制研究、疾病模型構(gòu)建或藥物干預(yù)實(shí)驗(yàn)中動(dòng)態(tài)監(jiān)測(cè)補(bǔ)體激活狀態(tài)，也可用于體外培養(yǎng)體系中補(bǔ)體相關(guān)生物標(biāo)志物的分析，為免疫調(diào)節(jié)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    C3c
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, urine, saliva
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C3c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5000Average %88
    Range %84-92
    1:10000Average %94
    Range %90-98
    1:20000Average %99
    Range %95-104
    1:40000Average %102
    Range %96-108
  • 回收率：
    The recovery of human C3c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8993-96
    EDTA plasma (n=4)10095-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.266 2.345 2.306 2.174
    201.946 1.973 1.960 1.828
    101.510 1.546 1.528 1.396
    51.185 1.198 1.192 1.060
    2.50.854 0.814 0.834 0.702
    1.250.521 0.501 0.511 0.379
    0.6250.335 0.325 0.330 0.198
    00.131 0.133 0.132 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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