Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

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Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

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中文名稱：

人α-N-乙酰氨基半乳糖苷酶(NAGA)酶聯免疫試劑盒
貨號：

CSB-EL015414HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
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產品描述：

人α-N-乙酰氨基半乳糖苷酶(NAGA)酶聯免疫試劑盒（CSB-EL015414HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、唾液、組織勻漿樣本中的NAGA含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，本產品適用于基礎醫學研究、代謝通路分析或疾病模型構建等領域，支持多類型樣本檢測，為探索NAGA在糖基化修飾、溶酶體功能調控及代謝相關疾病中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NAGA ELISA Kit; Alpha-N-acetylgalactosaminidase ELISA Kit; EC 3.2.1.49 ELISA Kit; Alpha-galactosidase B ELISA Kit
縮寫：

NAGA
Uniprot No.：

P17050
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, saliva, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NAGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	92-101
1:4	Average %	86
	Range %	82-90
1:8	Average %	105
	Range %	100-109

回收率：

The recovery of human NAGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.246	2.152	2.199	1.980
20	1.892	1.815	1.854	1.635
10	1.465	1.504	1.485	1.266
5	1.127	1.179	1.153	0.934
2.5	0.874	0.836	0.855	0.636
1.25	0.541	0.568	0.555	0.336
0.625	0.362	0.349	0.356	0.137
0	0.221	0.217	0.219

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
基因功能參考文獻：
1. results demonstrate the utility of eQTL mapping in the identification of novel asthma genes and provide evidence for the importance of FADS2, NAGA, and F13A1 in the pathogenesis of asthma. PMID: 24934276
2. the active sites of human lysosomal enzymes alpha-galactosidase and alpha-N-acetylgalactosaminidase have interconvertible specificites PMID: 20444686
3. Tumor cell alpha-N-acetylgalactosaminidase activity and its involvement in GcMAF-related macrophage activation. PMID: 12062184
4. Structural analysis revealed biochemical and phenotypic differences in these Kanzaki patients with the R329Q and R329W mutation. PMID: 15619430
5. NAGA mutation p.D217N (c.649G>A) in exon 6 and mutation p.E325K (c.973G>A) in exon 8 may have roles in alpha-N-acetylgalactosaminidase deficiency with cardiomyopathy PMID: 17171432
6. Specific enzymatic activity of serum alpha-N-acetylgalactosaminidase was significantly increased in stage III melanoma patients, but not in early stages. PMID: 19394758
7. The active site of human alpha-NAGAL has anomeric selectivity for its catalytic product and the structure reveals a novel active-site rearrangement upon hexose ligand binding. PMID: 19683538
8. Use of a modified NAGA in the development of enzyme replacement therapy for Fabry disease is reported. PMID: 19853240
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相關疾病：

Schindler disease (SCHIND); Kanzaki disease (KANZD)
亞細胞定位：

Lysosome.
蛋白家族：

Glycosyl hydrolase 27 family
數據庫鏈接：

HGNC: 7631

OMIM: 104170

KEGG: hsa:4668

STRING: 9606.ENSP00000379680

UniGene: Hs.75372

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.