Human 8-iso prostaglandin F2α,8-iso-PGF2a ELISA Kit

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人8異前列腺素F2α（8-iso-PGF2a）酶聯(lián)免疫試劑盒（CSB-E12100h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的8-iso-PGF2a含量。試劑盒檢測范圍為125 pg/mL-5000 pg/mL，適用于細(xì)胞氧化損傷機制研究、抗氧化藥物篩選評價以及環(huán)境或營養(yǎng)因素對機體氧化應(yīng)激影響的科學(xué)探索，為氧化應(yīng)激相關(guān)領(lǐng)域的基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)提供高效檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

8-iso prostaglandin F2α,8-iso prostaglandin F2 alpha,8-iso-PGF2α,8-iso-PGF2 alpha
縮寫：

8-iso-PGF2a
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

125 pg/mL-5000 pg/mL
靈敏度：

125 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 8-iso-PGF2a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	92
	Range %	85-99
1:4	Average %	95
	Range %	90-103
1:8	Average %	94
	Range %	90-99

回收率：

The recovery of human 8-iso-PGF2a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-103