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Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

  • 中文名稱:
    人8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒
  • 貨號:
    CSB-E10140h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒(CSB-E10140h)為競爭法ELISA試劑盒,定量檢測血清、尿液樣本中的8-OHdG含量。8-OHdG是細胞內DNA氧化應激損傷的標志性產物。其背景在于機體受內外部因素影響產生氧化應激,損傷DNA生成8-OHdG。研究機制方面,常檢測其水平以評估氧化應激程度,在疾病早期診斷、病情監測及藥物研發等領域有重要意義。試劑盒檢測范圍為 3.12 ng/mL-800 ng/mL,該產品適用于體外定量評估生物樣本中的氧化損傷水平,為探索氧化應激與疾病關聯機制、藥物篩選及毒理學研究提供高效工具,尤其適合需要高靈敏度和寬線性范圍的科研場景,滿足血清和尿液等易獲取樣本的便捷檢測需求。在衰老、腫瘤發生、神經退行性疾病及環境毒理等研究領域具有重要科研價值。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    8-OHdG
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine
  • 檢測范圍:
    3.12 ng/mL-800 ng/mL
  • 靈敏度:
    3.12 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-91

    1:2

    Average %

    87

    Range %

    81-95

    1:4

    Average %

    88

    Range %

    82-95

    1:8

    Average %

    86

    Range %

    81-92

  • 回收率:

    The recovery of human 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    84-96

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    800

    0.337

    0.344

    0.340

    0.340

    200

    0.873

    0.857

    0.865

    0.865

    50

    1.302

    1.328

    1.315

    1.315

    12.5

    1.784

    1.699

    1.741

    1.741

    3.12

    2.190

    2.291

    2.240

    2.240

    0

    2.534

    2.453

    2.493

    2.493

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
        8-OHdG是DNA氧化損傷的標志物,最新研究表明,其與各種疾病的發生和發展有關,包括癌癥、糖尿病、阿爾茨海默病等。此外,8-OHdG的水平也可以用作生活方式和環境暴露的生物標志物。


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