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  4. Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

  • 中文名稱：
    人5羥二十碳四烯酸(5-HETE)酶聯免疫試劑盒
  • 貨號：
    CSB-E17006h
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人5羥二十碳四烯酸(5-HETE)酶聯免疫試劑盒（CSB-E17006h）檢測血清、血漿樣本中的5-HETE含量。5-HETE即5-羥二十碳四烯酸，是花生四烯酸經5-脂氧合酶代謝的產物。它在炎癥、過敏等病理過程中扮演重要角色。研究機制主要圍繞其在細胞信號傳導中的作用，能調節免疫細胞活性、促進血管生成等，是藥物研發潛在靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產品適用于體外定量分析生物樣本中5-HETE的表達水平，為研究炎癥相關疾病機制、評估藥物干預效果或探索代謝通路調控提供可靠的實驗工具，廣泛應用于生命科學基礎研究、藥理學研究及生物標志物開發等領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    5-HETE
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Biochemicals
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 89  
    Range % 84-94  
    1:200 Average % 92  
    Range % 87-99  
    1:400 Average % 93  
    Range % 85-97  
    1:800 Average % 97  
    Range % 90-104  
  • 回收率：
    The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 91 84-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.739 2.652 2.696 2.586  
    1000 2.258 2.176 2.217 2.107  
    500 1.513 1.504 1.509 1.399  
    250 0.805 0.742 0.774 0.664  
    125 0.434 0.413 0.424 0.314  
    62.5 0.275 0.261 0.268 0.158  
    31.25 0.182 0.179 0.181 0.071  
    0 0.112 0.107 0.110 　  
  • 數據處理：
  • 貨期：
    3-5 working days

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