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Horse growth hormone,GH ELISA Kit

  • 中文名稱:
    馬生長(zhǎng)激素(GH)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E11242Eq
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    25 ng/mL-400 ng/mL
  • 靈敏度:
    25 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4800/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    馬生長(zhǎng)激素(GH)酶聯(lián)免疫試劑盒(CSB-E11242Eq)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GH1含量。GH1 為生長(zhǎng)激素 1 基因,其表達(dá)產(chǎn)物生長(zhǎng)激素與人體生長(zhǎng)發(fā)育密切相關(guān)。研究機(jī)制方面,它主要通過(guò)與受體結(jié)合,激活多種信號(hào)通路,促進(jìn)細(xì)胞增殖、分化和蛋白質(zhì)合成。異常的 GH1 表達(dá)或功能可能引發(fā)如巨人癥、侏儒癥等生長(zhǎng)異常疾病。試劑盒檢測(cè)范圍為25 ng/mL-400 ng/mL,適用于科研領(lǐng)域探究馬匹生長(zhǎng)調(diào)控、營(yíng)養(yǎng)代謝、繁殖性能及疾病模型中的GH動(dòng)態(tài)變化。適用于實(shí)驗(yàn)室對(duì)馬科動(dòng)物生理病理機(jī)制研究、激素相關(guān)藥物開(kāi)發(fā)評(píng)估以及畜牧領(lǐng)域GH水平監(jiān)測(cè)等科研需求本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Equus caballus (Horse)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of horse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %87
    Range %84-90
    1:4Average %96
    Range %90-102
    1:8Average %90
    Range %85-96
  • 回收率:
    The recovery of horse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9691-101
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    4000.202 0.204 0.203
    2000.356 0.346 0.351
    1000.597 0.582 0.590
    501.102 1.104 1.103
    252.319 2.354 2.337
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能參考文獻(xiàn):
    1. Plasma levels of growth hormone (as well as glucose, insulin, and leptin) are highly correlated with the duration of winter anovulatory phase. PMID: 21958120
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫(kù)鏈接: