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Horse Prolactin(PRL) ELISA kit

  • 中文名稱:
    馬催乳素(PRL)酶聯免疫試劑盒
  • 貨號:
    CSB-EL018724HO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    馬催乳素(PRL)酶聯免疫試劑盒(CSB-EL018724HO)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的PRL含量。PRL即泌乳素,是腺垂體分泌的多肽激素。其分泌受下丘腦調節。研究機制上,它在乳腺發育、泌乳啟動維持等生理過程中發揮重要作用,異常分泌會致月經不調、不孕等,在腫瘤發生發展等方面也有一定關聯,是醫學研究重要靶點。試劑盒檢測范圍為2.5 ng/mL-1000 ng/mL,產品經過嚴格的性能驗證,可穩定應用于馬科動物繁殖生理、泌乳調控機制、應激狀態評估等研究領域,為畜牧科研、動物內分泌研究及生殖健康管理提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Equus caballus (Horse)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    2.5 ng/mL-1000 ng/mL
  • 靈敏度:
    2.5 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-101
    1:2 Average % 95
    Range % 85-100
    1:4 Average % 91
    Range % 89-99
    1:8 Average % 93
    Range % 90-100
  • 回收率:
    The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-105
    EDTA plasma (n=4) 97 88-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    0 2.133 2.121 2.127
    2.5 1.516 1.501 1.509
    8.75 0.996 1.002 0.999
    31.25 0.538 0.555 0.547
    187.5 0.341 0.339 0.340
    1000 0.162 0.159 0.161
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

我想要確定這個CSB-EL018724HO Horse Prolactin(PRL) ELISA kit是否適用于犀牛樣品?用什么抗原來制造捕獲抗體?捕獲抗體是單克隆的還是多克隆的?競爭性抗原是什么?

A:
CSB-EL018724HO酶標板上包被山羊抗兔抗體(免疫原為兔IgG)。試劑盒中使用的特異性抗體為兔多克隆抗體。免疫原是一種CHO衍生的重組蛋白。標準品為CHO衍生的重組蛋白。對于犀牛樣品,我們沒有使用牛或馬的ELISA試劑盒進行測試。如果您想試一試,我們建議你先做一個預實驗。

靶點詳情

  • 功能:
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Somatotropin/prolactin family
  • 數據庫鏈接: