Goat growth hormone,GH ELISA KIT

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

山羊生長激素(GH)酶聯免疫試劑盒（CSB-E13275G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 編碼生長激素，其對人體生長發育至關重要。研究機制方面，GH1 表達產物生長激素主要通過與受體結合，激活 JAK-STAT 等信號通路，來調節細胞增殖、分化和代謝，影響骨骼、肌肉等組織生長，目前圍繞它的研究有望為生長相關疾病治療提供思路。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，本產品適用于科研領域，如動物生理學研究、內分泌機制探索、畜牧業生長調控優化或藥物開發中GH相關生物標志物分析，其檢測可為研究山羊生長發育、代謝疾病模型建立及營養干預效果評估提供重要依據。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P67931
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	87
	Range %	84-90
1:4	Average %	95
	Range %	91-99
1:8	Average %	92
	Range %	88-97

回收率：

The recovery of goat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	94	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻：
1. genetic variation and milk production and composition PMID: 23611522
亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: chx:100861230

UniGene: Chi.38639